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. Author manuscript; available in PMC: 2016 Oct 25.
Published in final edited form as: Nature. 2016 Apr 25;533(7602):255–259. doi: 10.1038/nature17626

Figure 2. B. ovatus does not require surface digestion for utilization of inulin.

Figure 2

a, Predicted inulin utilization locus of B. ovatus (Bo). Gene designations shown are preceded by BACOVA_. The color coding of gene products is as in Fig.1. b, Thin layer chromatography (TLC) analysis of inulin defined medium incubated for the indicated times with purified His-tagged 04502, 04503 or 04502 and 04503, or vector control. See SI Figure 1 for uncropped scanned images. c, Growth of Bo WT and mutants in inulin defined medium. d, TLC analysis of conditioned media during the growth of Bo WT and mutants in 0.5% and 0.1% inulin. EL = early log, ML = mid log, LL = late log, St = stationary. e, Growth of Bo WT, Δ04502/3, Δ04504 (susD ortholog) and Δ04505 (susC ortholog) mutants in inulin defined medium. For c,e, each line represents n=1 sample per condition. See Extended Data Fig.2 for additional independent experiments. In all panels error bars represent standard error; p values derived from two-tailed Student's t test.