Skip to main content
. Author manuscript; available in PMC: 2016 Oct 25.
Published in final edited form as: Nature. 2016 Apr 25;533(7602):255–259. doi: 10.1038/nature17626

Extended Data Figure 2. Bo 04502 and 04503 mutants grow equivalently to wild type on limiting concentrations of inulin and do not require surface digestion for utilization of inulin.

Extended Data Figure 2

a, Growth of Bo WT, Δ04502, Δ04503 and Δ04502/3 mutants in varying concentrations of inulin as indicated. Biological replicates of each condition are plotted as individual lines (n=2 cell culture biological replicates). Upper and lower panels are independent experiments. b, Independent experiments for Fig. 2d. EL = early log, ML = mid log, LL = late log, St = stationary phase. See SI Figure 1 for uncropped scanned images. c, Complementation of Bo Δ04502 and Δ04502/3 mutants with the respective genes in trans. TLC analysis of conditioned media from Bo Δ04502/3 (left panel) complemented in trans with BACOVA_04502, BACOVA_04503, BACOVA_04502/3 or vector alone (pFD340) and Bo Δ04502 (right panel) with BACOVA_04502 or vector alone grown in defined inulin media. See SI Figure 1 for uncropped scanned images. S = stationary phase.