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Comparison of fluorescence dyes and SERS NPs for ratiometric imaging.
| Properties | Fluorescence dyes | SERS NPs |
|---|---|---|
| Chemical composition | different | same |
| Excitation and/or emission wavelength range | different | same |
| Photon distribution in tissue | wavelength-dependent | same |
| Tissue autofluorescence | wavelength-dependent | same |
| Photostability | bleaches over time | stable |
| Multiplexing capability | typically 2–4 | >10 |
