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. 2016 Jul 28;173(17):2622–2632. doi: 10.1111/bph.13541

Figure 2.

Figure 2

TM5441 normalizes the alterations in lipid metabolism‐related enzymes induced by a HFD. (A and B) Western blotting analysis of HSL and ATGL in epididymal WAT lysates. (C–E) Total RNA was isolated from the epididymal WATs and subjected to quantitative reverse transcription PCR analysis to determine the expression of (C) HSL, (D) ATGL and (E) FAS. Expression was normalized against 18S rRNA levels. The data are shown as the mean ± SEM of seven mice. * P < 0.05 versus ND, † P < 0.05 versus HFD.