Figure 3.
TM5441 enhances insulin signalling and suppresses JNK phosphorylation in the epididymal WAT of HFD mice. (A) Insulin‐stimulated phosphorylation of Akt (Ser473) in the epididymal WAT was determined by western blot analysis. The three groups of mice were fasted overnight, before injection with insulin, and the epididymal WAT was removed 4 min after insulin injection. (B) Phosphorylation of JNK in the epididymal WAT was determined by western blot analysis. The data were shown as representative western blots and the mean ± SEM of seven mice. * P < 0.05 versus ND, † P < 0.05 versus HFD.