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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Mol Microbiol. 2016 Jun 2;101(4):606–624. doi: 10.1111/mmi.13411

Fig. 6.

Fig. 6

Effects of ylbF, ymcA and yaaT knockouts on expression of the tapA operon. (A–D) Luciferase assays for PtapA-luc expression were performed as described in Experimental procedures. The blue line at the bottom of panel C contains data for the ymcA and ymcA spo0F strains. (A) The effects of yaaT, ylbF and ymcA knockouts on a PtapA-luc expression reporter. (B) The effect of sinR inactivation on tapA expression in the ylbF, ymcA and yaaT knockouts. (C) and (D) show the extent of bypass of a ymcA null mutant by sof-1 and sad-67 respectively. The solid and dashed lines in Panel D show results with and without the addition of IPTG, respectively. The strains used were as follows. (A) wild-type ΔepsH PtapA-luc (BD7870), ΔymcA ΔepsH PtapA-luc (BD7872), ΔyaaT ΔepsH PtapA-luc (BD7871), ΔylbF ΔepsH PtapA-luc (BD7873). (B) wild-type ΔsinR ΔepsH PtapA-luc (BD7900), ΔymcA ΔsinRΔ epsH PtapA-luc (BD7974), ΔylbFΔ sinR ΔepsH PtapA-luc (BD7975), ΔyaaT ΔsinRΔ epsH PtapA-luc (BD7973). The strains used in panels A and B were deleted for epsH to avoid the clumping that occurs in sinR strains. We have shown that the elimination of epsH has no discernible effect on the expression of PtapA-luc (not shown). (C) wild-type PtapA-luc (BD7644), ΔymcA PtapA-luc (BD7652), ΔymcA Δspo0F sof-1 PtapA-luc (BD7654), Δspo0F sof-1 PtapA-luc (BD7651), Δspo0F Ptap -luc (BD7650). Panel (D) wild-type sad-67 PtapA-luc (BD7844), Δspo0F sad-67 PtapA-luc (BD7845), ΔymcA sad-67 PtapA-luc (BD7846). (E) Cells were grown in LB media until an OD600 of 1.0, and 5 μl were spotted on MSgg agar plates. Pictured is the colony morphology after four days of growth at room temperature. The scale bar corresponds to 1 cm and all the colonies were photographed at the same magnification. The sinR+ strains used were BD7736 (ΔymcA), BD7737 (ΔylbF), BD7731 (ΔyaaT) and BD7477 (wild-type). The sinR strains were BD7837 (ΔymcA), BD7838 (ΔylbF), BD7836 (ΔyaaT) and BD7835 (ΔsinR). Growth was in MSgg and all the strains were constructed in the 3610 background.