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. Author manuscript; available in PMC: 2017 Aug 3.
Published in final edited form as: Neuron. 2016 Aug 3;91(3):602–614. doi: 10.1016/j.neuron.2016.07.003

Figure 4.

Figure 4

Effects of bilateral preBötC photostimulation in SST-ChR2 mice after pharmacological injection of SST receptor antagonist CSST or bicuculline and strychnine (B+S). A, In SST-ChR2 mice, SST antagonism did not eliminate apnea induced by bilateral preBötC LPP. A1: f, TI, and TE measured before (Ctrl) and after (CSST) administration of CSST (*, P<0.05; n=4). A2: Bilateral preBötC injection of CSST did not eliminate apnea induced by bilateral preBötC LPP. Scale bar, 2 s. A3: Duration of bilateral preBötC LPP-induced apnea after administration of CSST compared to TE of previous cycle (*, P<0.05; n=4). B, In SST-ChR2 mice, B+S eliminated the bilateral preBötC LPP-induced apnea and SPP-induced prolongation in expiratory duration. B1: Bilateral preBötC SPP during midexpiration in B+S in SST-ChR2 mice triggered an ectopic inspiratory burst. Scale bar, 2 s. B2: Bilateral preBötC LPP in B+S in SST-ChR2 mice no longer produced apnea. Scale bar, 2 s. B3: Bilateral preBötC LPP in B+S in SST-ChR2 mice did not change TI or TE. C, Phase response curves show bilateral preBötC SPP in SST-ChR2 mice before block of inhibition (SST-ChR2: Ctrl, dark blue) led to phase delays that became phase advances after block of inhibition (SST-ChR2: B+S, light blue). *, ** comparisons versus control phase shift (Ctrl), P<0.05; +, comparisons of the phase shift before and after B+S injection, P<0.05. Error bars represent mean±SD.