Figure 4. CPI-203 inhibits the production of Th17 cytokines and Th17 downstream chemokines and cytokines in vivo.

PAO1 was grown to log phase and then inoculated at a dose of 10⁶ CFU by oropharyngeal inoculation into male C57Bl/6 mice (n = 5 per group). Mice were randomized to receive vehicle (Veh) or CPI-203 (CPI) (2.5 mg/kg) 60 minutes prior to infection and BID afterwards. (A) Lung bacterial burdens 24 hours after infection from control vehicle- and CPI-treated mice were determined by CFU and (B) BAL cell numbers were determined by quick-diff stain. (C) Gene expression in the lung was determined by RT-PCR and (D) total IL-17– and IFN-γ–producing cells were determined by intracellular staining. (E) Proinflammatory chemokines and cytokines in the whole-lung homogenates were determined by Luminex. P values were calculated by student t test.