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. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: Methods Enzymol. 2016 Jun 28;577:287–318. doi: 10.1016/bs.mie.2016.05.017

Scheme 2.

Scheme 2

Isotopic labeling for measuring KIEs on the hydride transfer (top) and the proton abstraction (bottom). The hydride (red) and the proton (green) are the sites of isotopic labeling. The asterisks on dUMP represent remote 14C labeling to trace lighter isotopes.