A: Raw tracings showing mEPSCs during control, application of 100 μM DEA/NO, washout, and 20 μM CNQX in a labeled NTS neuron. B and C: Cumulative plot analysis of mEPSCs of the same neuron showing the distributions of the inter-event interval (B) and amplitude (C) during control, application of DEA/NO, and washout. D: Superimposed averages of 100 consecutive mEPSCs obtained during control, application of DEA/NO, and washout. E and F: summary data showing that DEA/NO increased the frequency, but not amplitude, of mEPSCs in second order NTS neurons. Data were presented as means ± SEM. * P < 0.05 compared with control value.