Fig. 4.
Loss of Smoothened from the SHF leads to reduction of Lef1 expression in the pSHF. Sections from the venous pole of Smofl/fl control and SHF-Smofl/fl littermates at ED10.5 and ED13 were labeled for the expression of the Wnt/β-catenin target gene Lef1 (red). A,B: This analysis revealed reduction of Lef1 in SHF cells of SHF-Smofl/fl ckos (B) when compared with control littermates (A). C,D: Decreased Lef1 expression was also noted around the trachea of SHF-Smofl/fl ckos at later developmental stages. DMP, dorsal mesenchymal protrusion; SHF, second heart field; Tr, trachea