Fig. 5.
Deleting Smoothened from the SHF results in a reduction of Axin2 in the pSHF. A–D: Sections from the venous pole of Smofl/fl control (A,C) and SHF-Smofl/fl (B,D) littermates at ED10.5 were stained for Axin 2 (red). C: Higher magnification of boxed area in A. D: Higher magnification of boxed are in B. The immunofluorescently labeling showed reduced expression of Axin 2 in the pSHF of SHF-Smofl/fl cko embryos (B,D) when compared with control littermates (A,C). Blue staining in C and D is DAPI nuclear stain. FG, foregut; pSHF, posterior second heart field