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Membrane curvature induced by RNCL-based remodeling. GUVs composed of neutral phospholipid 3 containing 0.1 mol % of Bodipy FL DHPE dye are remodeled by RNCL exchange reactions with negatively charged lysolipid 15, leading to the formation of a new class of GUVs composed of 16. After addition of amphiphysin to the mixture, dramatic membrane curvature events in the negatively charged GUVs of 16 were observed as a result of the protein binding. (Scale bar, 5 µm.)
