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Effects of Gfh factors on intrinsic transcription termination by Dra RNAP. (A) Scheme of the template. Positions of the T7A1 promoter, 20-nt U-less region, λ phage tR2 terminator, and run-off RNA are shown. (B) Analysis of transcription termination was performed in the presence of 10 mM Mg2+ or Mn2+ ions at 37 °C. Termination efficiencies are shown as the percentage of the terminated (tR2) transcripts relative to the sum of tR2 and RO RNAs.
