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Effects of Gfh factors on transcription initiation. (A) Sequence of the T7A1cons promoter used in the abortive initiation assay. (B) Inhibition of abortive transcription by Gfh1 and Gfh2 factors from Dra at different pH values in the presence of Mg2+ (Left) or Mn2+ (Right). Transcription was performed with the CpA primer and UTP at 37 °C. RNAP activity is shown as a percentage of the CpApU synthesis measured in the absence of Gfh factors.
