Fig. S8.

Analysis of the effects of Gfh factors on Rho-dependent transcription termination by Dra RNAP. (Upper) Scheme of the template. The λ phage P_R promoter, 37-nt C-less region, 400-nt region containing putative trpt′ terminator (term.), and run-off RNA (RO) are indicated. (Lower) Reactions were performed in the presence of 10 mM Mn²⁺ ions at 20 °C; Gfh factors and Rho were added to 5 μM and 1 μM, respectively. The position of the termination region is shown by a vertical line on the left of the gel.