Fig. S7.

Agarose gel picture depicting precursor forms (unspliced pre-mRNA) and mature forms (fully-spliced mRNA) of GAPDH mRNA from nuclear and cytoplasmic fractions of CD4⁺ T cells freshly isolated from a healthy volunteer. The GAPDH pre-mRNA primer pair produced a 207-bp fragment corresponding to exon 7–intron 7 of the human GAPDH gene. The GAPDH mRNA primer pair produced two bands: a 197-bp fragment corresponding to exon 4 and exon 5 of the GAPDH gene; and a 328-bp fragment corresponding to exon 4 and exon 5 that included 131 bp of intron sequence.