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TAC65 is essential in bloodstream form cells (NYsm) but dispensable in an engineered bloodstream from a cell line that can grow in the absence of the kDNA (F1γL262P). Graphs show growth curves and loss of kDNA of the indicated bloodstream form cell lines. Red lines depict percentage of cells still having the kDNA. (Insets) Northern blots demonstrating ablation of endogenous TAC65 mRNA. The corresponding EtBr-stained rRNAs serve as loading controls.
