Fig. S1.
Complementation of L. pneumophila Paris lpp0148TAA mutant. (A) Northern blot analysis of comEA expression in the Paris wild-type strain and lpp0148TAA mutant transformed with the empty plasmid pX3, the pX3 plasmid carrying the lpp0148 gene alone (pX3-0148), or the putative lpp0148-0149 operon (pX3-0148-9). (B) Determination of the transformation frequency of the same strains. Two clones for each of the complementing plasmids were tested (#1 and #2). Bacteria were exposed to transforming DNA and then plated on selective and nonselective media. The transformation frequency is the ratio of the number of cfus counted on selective medium divided by the number of cfus counted on nonselective medium. Transformation frequency for the Paris strain was below the detection limit (<1.10−9). Error bars represent SE of experimental determination of cfu counts.