Table S1.
Comparison between the L. pneumophila Paris wild-type strain and its lpp0148TAA mutant derivative by RNA-seq analysis
| RNA-seq lpp0148TAA versus WT (fold change > 2; Padj < 0.01) | |||
| Feature | Fold change | Padj | Name and/or function |
| lpp2554 | 10.54 | 3.6E-147 | Hypothetical |
| lpp0872 | 7.99 | 2.0E-168 | comEA |
| lpp0640 | 7.13 | 1.9E-208 | comM |
| lpp2553 | 3.82 | 2.4E-50 | radC |
| lpp2280 | 3.14 | 1.4E-41 | comF |
| lpp2555 | 2.97 | 8.5E-51 | lepB |
| lpp0639 | 2.86 | 1.8E-32 | Hypothetical |
| lpp0873 | 2.51 | 1.6E-34 | mreB |
| lpp0680 | 2.18 | 3.3E-13 | comEC |
| lpp0851 | 2.08 | 8.8E-14 | Hypothetical; transposase IS200 like |
| lpp1976 | 2.06 | 1.5E-14 | Hypothetical; pilin homolog |
| lpp0712 | −2.03 | 8.6E-11 | Hypothetical |
| lpp0148 | −3.96 | 2.1E-53 | lpp0148 (ProQ/FinO domain) |
| lpp0149 | −6.65 | 1.2E-85 | Hypothetical |
Total RNA from bacterial cultures grown to an OD600 of 0.8 at 37 °C was extracted, purified, and sequenced (Illumina). This was done three times for each strain. The number of reads was normalized, and the statistical significance (Padj) of the obtained enrichment was calculated as described in SI Materials and Methods.