Table S2.

Comparison between RNAs coimmunoprecipitated using an anti-Lpp0148 antibody in the L. pneumophila Paris wild-type strain and its lpp0148_TAA mutant derivative by RIP-seq analysis

RIP-seq WT versus lpp0148TAA; enriched RNA with Padj < 0.01
Feature	Start position	Normalized counts		Fold enrichment	Padj
		Average in WT	Average in lpp0148TAA
lppnc0692 (rocR)	3384521	718,134.1	3,356.3	214	2.3E-12
lppnc0344	1593477	24.8	0.4	69	1.3E-4
lpp2366	2713176	85.7	2.2	40	6.2E-3
lppnc0319	1498044	8.4	0.4	23	4.0E-4
lppnc0187	863098	8.7	0.6	15	7.0E-3

At an OD₆₀₀ of 0.8 exponentially growing bacterial cultures were fixed with 1% formaldehyde, and Lpp0148 and its partners were immunoprecipitated using a specific antibody. RNA was extracted, purified, and sequenced (Illumina). This was repeated three times in the WT strain and two times in the lpp0148_TAA strain. The number of reads was normalized, and the statistical significance (P_adj) of the obtained enrichment was calculated as described in SI Materials and Methods.