Table S3.
Bacterial strains and plasmids used in this study
| Strains/plasmids | Relevant genotype | Reference |
| Strains | ||
| L. pneumophila | ||
| JR32 WT | Philadelphia-1 derivative; SmR; r− m+ | (44) |
| JR32 Δlpp0148 | JR32; Δlpp0148 | This study |
| JR32 ΔrocR | JR32 ΔrocR | This study |
| JR32 ΔrocR::MK | JR32 ΔrocR::(lacIq, Ptac-mazF, nptII); KanR | This study |
| JR32 rocRm3 | JR32 rocRm3 | This study |
| JR32 rocRm4 | JR32 rocRm4 | This study |
| JR32 rocRm5 | JR32 rocRm5 | This study |
| LELA3825 | JR32; lpp0148::(Tn903dIIlacZ::nptII); KanR | (34) |
| LELA3825G | JR32; lpp0148::(Tn903dIIlacZ::aacC1); GentR | This study |
| Paris WT | Paris Outbreak isolate CIP107629 | CNR Lyon |
| Paris lpp0148TAA | Paris; lpp0148TAA | (22) |
| Paris ΔrocR | Paris; ΔrocR | This study |
| Paris ΔrocR::MK | Paris; ΔrocR::(lacIq, Ptac-mazF, nptII); KanR | This study |
| E. coli | ||
| DH5α, λpir | F−, supE44, ΔlacU169 (ΦlacZΔM15), recA1, endA1, hsdR17, thi-1, gyrA96, relA1, λpir lysogen | Laboratory strain collection |
| BL21(DE3) | F−, ompT, gal, dcm, lon, hsdSB(rB- mB-), λ[DE3 (lacI, lacUV5-T7 gene 1, ind1, sam7, nin5)] | Laboratory strain collection |
| Plasmids | ||
| pET-15b_Lpp0148aa1–230 | pET-15b(+) derivative; IPTG-inducible production of His-Lpp0148aa1–230 (Paris); AmpR | This study |
| pET-47b_Lpp0148aa1–230 | pET-47b(+) derivative; IPTG-inducible production of His-(HRV-3C)-Lpp0148aa1–230 (Paris); KanR | This study |
| pGEM-ihfB::Kan | pGEM-T Easy; ihfB::nptII; AmpR, KanR | (22) |
| pGEM-MK | pGEM-T Easy; lacIq, Ptac-mazF, nptII; AmpR, KanR | This study |
| pGEM-SK | pGEM-T Easy; sacB-mazF, nptII; AmpR, KanR | This study |
| pGEMPKD4 | pGEM-T Easy; FRT- nptII -FRT; AmpR, KanR | This study |
| pGEX-6P-1_Lpp0148aa1–126 | pGEX-6P-1 derivative; IPTG-inducible production of GST-(HRV-3C)-Lpp0148aa1–126 (Paris); AmpR | This study |
| pKD4 | pANTS-γ; FRT- nptII -FRT; AmpR, KanR | (35) |
| pLLA21 | pX3; ProcR-rocR*, cat; CmR | This study |
| pLLA27 | pXDC91 with mutated RocR box (m3: TttAAC); ΔmobA; PcomEAm3-comEA_gfp+, cat; CmR | This study |
| pLLA28 | pXDC91 with mutated RocR box (m4: TCCttC); ΔmobA; PcomEAm4-comEA_gfp+, cat; CmR | This study |
| pLLA29 | pXDC91 with mutated RocR box (m5: ctacAC); ΔmobA; PcomEAm5-comEA_gfp+, cat; CmR | This study |
| pX3 | RSF1010 derivative (replicative in L. pneumophila); ΔmobA; gfp+ (no promoter), cat; CmR | (12) |
| pX3_148 | pX3; Plpp0148-lpp0148†, cat; CmR | This study |
| pX3_148-9 | pX3; Plpp0148-lpp0148_lpp0149‡, cat; CmR | This study |
| pXDC46 | pGEM-T Easy; Tn903dIIlacZ::aacC1; AmpR, GentR | (34) |
| pXDC91 | RSF1010 derivative (replicative in L. pneumophila); ΔmobA; PcomEA-comEA§_gfp+, cat; CmR | (12) |
Abbreviations of antibioresistance (XxxR) are as follows: Amp, ampicillin; Cm, chloramphenicol; Gent, gentamycin; Kan, kanamycin; Sm, streptomycin.
*
Locus 3384431–3384710 from Paris strain.
†
Locus 165011–165943 from Paris strain.
‡
Locus 165011–167014 from Paris strain.
§
Locus 889182–889694 from JR32 strain.