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Determination of Nun/TEC Kd and GreA,B/TEC Kd for 3′ and 5′ RNA cross-links. (A) Quantitative analysis of Nun 5′ and 3′ cross-linking products with various Nun concentrations. (B and C) The same analysis for Nun cross-linking products in the presence of various concentrations of GreA (B) or GreB (C). Concentration of GreA/B proteins is indicated.
