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. 2016 Jul 20;113(31):8825–8830. doi: 10.1073/pnas.1603364113

Fig. 3.

Fig. 3.

Mapping the C termini of Gn protein by mutagenesis. (A) Mutations in the Gn CT and NSm domain I (on pTM1BUN-Gn332). * represents the stop codon. (B) Effect of deletions on the migration shift of Gn protein. (C) Profile of the radiolabeled intracellular and virion proteins. (D) The expression of Gn332 (preGn) and Gn308V5. (E) WB analysis of V5-tagged Gn332 at residue 27 or 86. (F) Cell fusion assay on BSRT7/5 cells cotransfected with pTM1BUN-NSmGc (Gc) and one of the Gn mutants. *P < 0.05; ****P < 0.0001.