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. 2016 Jul 20;113(31):8825–8830. doi: 10.1073/pnas.1603364113

Fig. 5.

Fig. 5.

Requirement of SPNSm for GPC processing, cell fusion, and virus replication. (A) Schematic showing either deletion of NSm or mutations in SPNSm. (B) Effect of mutations on the GPC cleavage. (C) Cell fusion on BSRT7/5 cells transfected with BUNM mutants. (D–F) The protein profile (D), plaque phenotype (E), and growth curve (F) of the recombinant virus lacking NSm (rBUN∆NSm). (G) Effect of deletion mutations in SPNSm on the migration shift of Gn protein. (H) Cell fusion on BSRT7/5 cells cotransfected with pTM1BUN-NSmGc and one of the Gn mutants. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.