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. 2016 Jul 20;113(31):8825–8830. doi: 10.1073/pnas.1603364113

Fig. S5.

Fig. S5.

shGFP lentivirus contains antivirus activity. (A) Biological IFN assay. A549/Npro cells were treated with series-diluted IFN-α (pbI assay science), lentivirus, or cell medium for 24 h and then challenged with EMCV (at MOI of 0.1) for 48 h or until obvious CPE occurred. Cells were fixed and stained with 4% formaldehyde/0.1% crystal violet solution. (B) Comparison of BUNV and PUUV infections responsive to shGFP lentivirus. A549/Npro cells were treated with different dilutions of shGFP lentivirus and infected with either BUNV or PUUV at MOI of 0.1. The result was shown as fold inhibition of virus titers between the induced and noninduced cells. *P < 0.05; **P < 0.01.