Table S3.
Effect of introducing AtSAL1 Cys167-Cys190 disulfide pair on redox sensitivity of ScHAL2
| Protein | [PAP], µM | Specific activity, pmol per µg protein per min | |||
| Reduced | Oxidized | Decrease in activity, % | Average decrease in activity, % | ||
| ScHAL2 | 3.35 | 124 ± 0.9 | 77 ± 16 | 38 | 46 ± 4.8a |
| 13.4 | 414 ± 66 | 220 ± 31 | 46 | ||
| 26.8 | 597 ± 150 | 270 ± 35 | 54 | ||
| ScHAL2+3C | 3.35 | 53 ± 10 | 17 ± 2 | 67 | 67 ± 1.4b (P < 0.005 relative to ScHAL2) |
| 13.4 | 72 ± 24 | 25 ± 5 | 65 | ||
| 26.8 | 81 ± 29 | 24 ± 0.8 | 70 | ||
Enzymatic assays were performed with 0.2 µg recombinant protein in the presence of 20 mM GSH (reduced) or GSSG (oxidized) at 30 °C. Means and SE from two independent experiments are shown. The decrease in activity for ScHAL2+3C by oxidation is significantly greater (a, b; P < 0.005) than that in WT ScHAL2.