Table 1.
Kinetic parameters | Monomeric AtSAL1 | Dimeric AtSAL1 | OsSAL1 | |||
Reduced | Oxidized | Reduced | Oxidized | Reduced | Oxidized | |
kcat, min−1 | 123 ± 18 | 119 ± 13 | 24.4 ± 1.1 | 4.3 ± 1.3 | 44 ± 3.9 | 11 ± 1.5 |
KM, µM PAP | 9.9 ± 3.3 | 8.3 ± 1.2 | 8.7 ± 5.2 | 4.2 ± 1.8 | 20 ± 6.9 | 61 ± 23.6 |
kcat/KM, µM−1⋅min−1 | 12.4 | 14.2 | 2.8 | 1.0 | 2.2 | 0.2 |
Enzymatic assays were performed at 25 °C with 0.2 µg recombinant AtSAL1 in the presence of 5 mM DTT (reduced) or DTTox (oxidized). Results shown are means and SE from two independent experiments. For OsSAL1, activity of 0.2 µg recombinant protein was assayed at 25 °C in the presence of 20 mM GSH (reduced) or GSSG (oxidized). Experiment was performed twice, with similar results.