. 2016 Jul 18;113(31):E4567–E4576. doi: 10.1073/pnas.1604936113

Table 1.

Enzyme kinetics under different redox states for two oligomeric forms of AtSAL1 and its orthologous rice protein, OsSAL1

Kinetic parameters	Monomeric AtSAL1		Dimeric AtSAL1		OsSAL1
Kinetic parameters	Reduced	Oxidized	Reduced	Oxidized	Reduced	Oxidized
k_cat, min⁻¹	123 ± 18	119 ± 13	24.4 ± 1.1	4.3 ± 1.3	44 ± 3.9	11 ± 1.5
K_M, µM PAP	9.9 ± 3.3	8.3 ± 1.2	8.7 ± 5.2	4.2 ± 1.8	20 ± 6.9	61 ± 23.6
k_cat/K_M, µM⁻¹⋅min⁻¹	12.4	14.2	2.8	1.0	2.2	0.2

Enzymatic assays were performed at 25 °C with 0.2 µg recombinant AtSAL1 in the presence of 5 mM DTT (reduced) or DTT_ox (oxidized). Results shown are means and SE from two independent experiments. For OsSAL1, activity of 0.2 µg recombinant protein was assayed at 25 °C in the presence of 20 mM GSH (reduced) or GSSG (oxidized). Experiment was performed twice, with similar results.