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Sequence alignment of the Ub-binding sites of the human USP family. The Ub-binding sites were defined as the 52 residues in USP2 that are within 4.5 Å of Ubv.2.1 in the structure of the USP2:Ubv.2.1 complex (PDB ID code 3V6C), and the alignment for these residues is shown for 48 human USPs. Residues are shaded based on bits score: green ≤2 (nonconserved), 2< yellow <2.7 (conserved), red ≥ 2.7 (highly conserved).
