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LptE is required for engagement of LptD with Bam. LptD4213 stalls on the Bam complex with LptE. WT LptD does not accumulate on BamA or BamD when LptE levels are limiting. Samples were subjected to α-LptD, α-LptE, and α-His immunoblot analyses after DSP cross-linking, affinity purification, and cleavage of cross-linkers in wt, lptD4213, lptD4213sup, and LptE-limiting strains expressing His-tagged BamA (A) or His-tagged BamD (B).
