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Expression and localization of SM+ Dnf1 mutations. N-terminal GFP-tagged Dnf1+, Dnf1N220S, Dnf1N220S,F1192L, and Dnf1N220S,L1202P all display robust PM localization and enrichment at the bud neck. GFP-Dnf1N220S,C564S does not exhibit robust expression or PM/bud neck localization. The representative images were selected from 10 acquisitions of 3 independent transformants. (Scale bar, 5 µm.)
