Table S1.
Plasmids used in this study
| Plasmid | Notes | Source |
| pRS313 | (60) | |
| pRS313-Dnf1 | (15) | |
| pRS313-Dnf1 I228T, D258E, T226A | Present study | |
| pRS313-Dnf1 L242S | Present study | |
| pRS313-Dnf1 D1246G, I1260V | Present study | |
| pRS313-Dnf1 Y1221F | Present study | |
| pRS313-Dnf1 F1192L | Present study | |
| pRS313-Dnf1 Y585F, V600F, V613F | Present study | |
| pRS313-Dnf1 C564S | Present study | |
| pRS313-Dnf1 F602L,Y618F | Present study | |
| pRS313-Dnf1 L1202P | Present study | |
| pRS313-Dnf1 K276D | Present study | |
| pRS313-Dnf1 N220S, L242S | Present study | |
| pRS313-Dnf1 V573A, A594P, F599I | Present study | |
| pRS313-Dnf1 D576G, I623V, I654F | Present study | |
| pRS313-Dnf1 L1202P | Present study | |
| pRS313-Dnf1 A1201V, L1202S | Present study | |
| pRS313-Dnf1 C564S | Present study | |
| pRS313-Dnf1 E166G, S263T | Present study | |
| pRS313-Dnf1 F1192L | Present study | |
| pRS313-Dnf1 V221I | Present study | |
| pRS313-Dnf1 H216R, N217D | Present study | |
| pRS313-Dnf1 L1202S | Present study | |
| pRS313-Dnf1 YIS-FVT | Dnf1 Y618F, I619V, S620T | (36) |
| pRS313-Dnf1 I654S | Present study | |
| pRS313-Dnf1 S567A | Present study | |
| pRS313-Dnf1 N220S | Present study | |
| pRS313-Dnf1 L242S | Present study | |
| pRS313-Dnf1 N220C | Present study | |
| pRS313-Dnf1 N220D | Present study | |
| pRS313-Dnf1 N220M | Present study | |
| pRS313-Dnf1 N220Q | Present study | |
| pRS313-Dnf1 N220R | Present study | |
| pRS313-Dnf1 N220S | Present study | |
| pRS313-Dnf1 N220T | Present study | |
| pRS313-Dnf1 F213S | (38) | |
| pRS313-Dnf1 T254A | (38) | |
| pRS313-Dnf1 D258E | (38) | |
| pRS313-Dnf1 GA-QQ | Dnf1 G230Q,A231Q | (39) |
| pRS313-Dnf1 N550S | (39) | |
| pRS313-Drs2 | (50) | |
| pRS313-Dnf1 N220S, L1202P | Present study | |
| pRS313-Dnf1 N220S, F1192L | Present study | |
| pRS313-Dnf1 N220S, L1202S | Present study | |
| pRS313-Dnf1 I615M | Present study | |
| pRS313-Dnf1 I615S | Present study | |
| pRS313-Dnf1 I615T | Present study | |
| pRS415-Lem3 | Beta subunit of Dnf1 | Present study |
| pRS416-GFP | Soluble GFP | (39) |
| pRS416-GFP-Dnf1 | N-terminal GFP fusion | (39) |
| pRS416-GFP-Dnf1 N220S | Present study | |
| pRS416-GFP-Dnf1 N220S, C564S | Present study | |
| pRS416-GFP-Dnf1 N220S, F1192L | Present study | |
| pRS416-GFP-Dnf1 N220S, L1202P | Present study |