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. 2016 Jul 18;113(31):E4460–E4466. doi: 10.1073/pnas.1525730113

Table S2.

Strains used in this study

Strain Genotype Plasmid Source
PFY3275F MATa his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 dnf1Δ dnf2Δ None (12)
ZHY615M2D MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 drs2Δ None (12)
ZHY704 MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 dnf1Δ dnf2Δ dnf3Δ drs2Δ::LEU2 pRS416-DRS2 pRS416-DRS2 (12)
BRY1400 PFY3275F pRS415-Lem3 Present study
BRY11 pRS313 PFY3275F pRS313 Present study
BRY11 Dnf1 PFY3275F pRS313-Dnf1 Present study
BRY1103 PFY3275F pRS313-Dnf1 I228T, D258E, T226A Present study
BRY1101 PFY3275F pRS313-Dnf1 L242S Present study
BRY1144 PFY3275F pRS313-Dnf1 D1246G, I1260V Present study
BRY1143 PFY3275F pRS313-Dnf1 Y1221F Present study
BRY1132 PFY3275F pRS313-Dnf1 F1192L Present study
BRY1130 PFY3275F pRS313-Dnf1 Y585F, V600F, V613F Present study
BRY1126 PFY3275F pRS313-Dnf1 C564S Present study
BRY1125 PFY3275F pRS313-Dnf1 F602L, Y618F Present study
BRY1140 PFY3275F pRS313-Dnf1 L1202P Present study
BRY1105 PFY3275F pRS313-Dnf1 K276D Present study
BRY1104 PFY3275F pRS313-Dnf1 N220S, L242S Present study
BRY1124 PFY3275F pRS313-Dnf1 V573A, A594P, F599I Present study
BRY1123 PFY3275F pRS313-Dnf1 D576G, I623V, I654F Present study
BRY1142 PFY3275F pRS313-Dnf1 L1202P Present study
BRY1141 PFY3275F pRS313-Dnf1 A1201V, L1202S Present study
BRY1114 PFY3275F pRS313-Dnf1 C564S Present study
BRY1109 PFY3275F pRS313-Dnf1 E166G, S263T Present study
BRY1133 PFY3275F pRS313-Dnf1 F1192L Present study
BRY1106 PFY3275F pRS313-Dnf1 V221I Present study
BRY1108 PFY3275F pRS313-Dnf1 H216R, N217D Present study
BRY1145 PFY3275F pRS313-Dnf1 L1202S Present study
BRY1128 PFY3275F pRS313-Dnf1 I654S Present study
BRY1129 PFY3275F pRS313-Dnf1 S567A Present study
BRY11YISFVT PFY3275F pRS313-Dnf1 YIS-FVT Present study
BRY1104.0 G PFY3275F pRS313 Present study
BRY1104.1 G PFY3275F pRS313-Dnf1 Present study
BRY1104.2 G PFY3275F pRS313-Dnf1 N220C Present study
BRY1104.3 G PFY3275F pRS313-Dnf1 N220D Present study
BRY1104.5 G PFY3275F pRS313-Dnf1 N220M Present study
BRY1104.6 G PFY3275F pRS313-Dnf1 N220Q Present study
BRY1104.7 G PFY3275F pRS313-Dnf1 N220R Present study
BRY1104.8 G PFY3275F pRS313-Dnf1 N220S Present study
BRY1104.9 G PFY3275F pRS313-Dnf1 N220T Present study
BRY11F213S PFY3275F pRS313-Dnf1 F213S Present study
BRY11T254A PFY3275F pRS313-Dnf1 T254A Present study
BRY11D258E PFY3275F pRS313-Dnf1 D258E Present study
BRY11N550S PFY3275F pRS313-Dnf1 N550S Present study
BRY1200 ZHY615M2D pRS313 Present study
BRY1201 ZHY615M2D pRS313-Dnf1 Present study
BRY1202 ZHY615M2D pRS313-Drs2 Present study
BRY1204 ZHY615M2D pRS313-Dnf1 N220S, L242S Present study
BRY1204.7 ZHY615M2D pRS313-Dnf1 N220R Present study
BRY1204.8 ZHY615M2D pRS313-Dnf1 N220S Present study
BRY12GAQQ ZHY615M2D pRS313-Dnf1 GA-QQ Present study
BRY12N550S ZHY615M2D pRS313-Dnf1 N550S Present study
BRY1204.8.14 ZHY615M2D pRS313-Dnf1 N220S, C564S Present study
BRY1204.8.32 ZHY615M2D pRS313-Dnf1 N220S, F1192L Present study
BRY1204.8.40 ZHY615M2D pRS313-Dnf1 N220S, L1202P Present study
BRY1404.0 BRY1400 pRS416-GFP Present study
BRY1404.1 BRY1400 pRS416-GFP-Dnf1 Present study
BRY1404.8 BRY1400 pRS416-GFP-Dnf1 N220S Present study
BRY1404.8.14 BRY1400 pRS416-GFP-Dnf1 N220S, C564S Present study
BRY1404.8.32 BRY1400 pRS416-GFP-Dnf1 N220S, F1192L Present study
BRY1404.8.40 BRY1400 pRS416-GFP-Dnf1 N220S, L1202P Present study
BRY1104.1 D PFY3275F pRS313-Dnf1 L1202P Present study
BRY1104.8 D PFY3275F pRS313-Dnf1 N220S, L1202P Present study
BRY1104.8.32 PFY3275F pRS313-Dnf1 N220S, F1192L Present study
BRY1104.8.45 PFY3275F pRS313-Dnf1 N220S, L1202S Present study
BRY11I615M PFY3275F pRS313-Dnf1 I615M Present study
BRY11I615S PFY3275F pRS313-Dnf1 I615S Present study
BRY11I615T PFY3275F pRS313-Dnf1 I615T Present study