Fig. 6.

Nuclear localization and translocation of ZFP106. (A) Confocal microscopy of COS7 cells showing coexpression and localization of GFP-ZFP106 with SC-35 or mCherry-RBM39. The N-terminal fragment of ZFP106 (ZFP106-N) localized to a nuclear speckle pattern that overlapped with SC-35 and an mCherry-RBM39 fusion protein. (B) Inhibition of total cellular RNA synthesis by DRB treatment induced the translocation of GFP-ZFP106 from nuclear speckles to the nucleolus. (C) After removal of DRB, GFP-ZFP106 regained a nuclear speckle pattern over the course of 50 min. (D) Coexpression of GFP-ZFP106 and mCherry-KNOP1 induced the translocation of ZFP106 from nuclear speckles to the nucleolus. (Scale bars, 5 µm.)