Fig. 4.
LegC2, LegC3, LegC7, and VAMP4 form SDS-resistant complexes. (A) Relative purity of the recombinant, purified LegC2, LegC3, LegC7, and VAMP4 proteins used in this study, illustrated as Coomassie-stained bands upon SDS/PAGE. Note that the strongly stained upper bands correspond to the expected Mr of the respective proteins. (B) Purified LegC2, LegC3, and LegC7 were incubated with or without VAMP4, followed by SDS/PAGE and Coomassie staining. The lower (smaller) arrowhead indicates VAMP4; the upper (bigger) arrowhead indicates the LegC–VAMP4 hybrid complex (band of slower mobility). (C) Percentage coverage of all four proteins derived from proteomic analysis of the latter band.