Fig. S2.

Crbn deficiency does not affect B-cell and Foxp3⁺CD4⁺ regulatory T-cell populations and B-cell activations. (A) Total spleen cells were isolated from Crbn^−/− and Crbn^+/− mice. B-cell populations, including immature B cells (B220⁺IgM⁺IgD⁻), mature B cells (B220⁺IgM⁺IgD⁺), follicular B cells (B220⁺CD21^lowCD23⁺), and marginal zone B cells (B220⁺CD21⁺CD23⁻), were analyzed by flow cytometry. (B) Total spleen cells were isolated from Crbn^−/− and Crbn^+/− mice, and the Foxp3⁺CD4⁺ regulatory T-cell population was examined by flow cytometry. (C) B cells were isolated from the spleen of Crbn^−/− mice and Crbn^+/− mice. Isolated B cells were stimulated with an anti-IgM Fab antibody (20 μg/mL) for the indicated times, and the cell surface expression of CD86 and CD69 was analyzed by flow cytometry. Data are representative of three independent experiments.