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. 2016 Jul 20;113(31):8771–8776. doi: 10.1073/pnas.1502166113

Fig. S3.

Fig. S3.

CRBN overexpression reduces anti-CD3/CD28–induced T cell activation. (A) Jurkat T cells overexpressing CRBN and control Jurkat T cells were activated with anti-CD3 and anti-CD28 antibodies for 24 h. The cell surface expression of activation markers (CD69 and CD25) was then analyzed by flow cytometry. (B) IL-2 mRNA expression and IL-2 production were analyzed by RT-PCR and ELISA, respectively. (C) The CRBN overexpression cassette was introduced into mouse primary CD4+ T cells using a recombinant retrovirus. The cells were then stimulated with anti-CD3 and anti-CD28 antibodies. The expression of activation markers (CD69 and CD25) was then analyzed after sorting of infected cells. (D) Expression of IL-2 mRNA and IL-2 production were analyzed by RT-PCR and ELISA, respectively. Data are representative of three independent experiments. Results are expressed as mean ± SD. **P < 0.01, unpaired two-tailed Student’s t test.