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CRBN deficiency does not affect IKZF1 and IKZF3 protein levels in CD4+ Tn and Tem cells. Total protein was isolated from CD4+ Tn and Tem cells of Crbn−/− and Crbn+/− mice. IKZF1 and IKZF3 protein levels in the isolated CD4+ Tn and Tem cells were analyzed by immunoblot analysis with anti-IKZF1 and anti-IKZF3 antibodies. GAPDH served as an internal protein loading control. Data are representative of three independent experiments.
