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. 2016 Jul 20;113(31):8771–8776. doi: 10.1073/pnas.1502166113

Fig. S6.

Fig. S6.

Down-regulation of Cul4A and EZH1 in CD4+ T cells increases Kv1.3 mRNA expression level in CD4+ T cells. Here CD4+ T cells were isolated from C57BL/6 mice and shRNA for Cul4A (Sc-60470-V; Santa Cruz Biotechnology) or EZH1 (Sc-38188-V; Santa Cruz Biotechnology) was introduced into isolated CD4+ T cells using recombinant lentiviruses. Scramble shRNA lentivirus (Sc-108080; Santa Cruz Biotechnology) served as the control. After the lentivirus-mediated shRNA delivery, CD4+ T cells were cultured for 3 d with IL-7 (10 ng/mL), and total cellular RNAs were isolated from the cells. With the isolated RNAs, Kv1.3 and Cul4A or Kv1.3 and EZH1 mRNA levels were analyzed by quantitative RT-PCR analysis. GAPDH served as an internal gene expression control. Data are representative of three independent experiments. The results are expressed as mean ± SD. **P < 0.01, unpaired two-tailed Student’s t test.