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CD4+ T cells from Crbn−/− mice show increased differentiation onto Th17 cells in vitro. CD4+ T cells were isolated from Crbn−/− and Crbn+/− mice and differentiated into Th17 cells as described in the text. IL-17A–producing CD4+ T cells among the in vitro-differentiated Th17 cell population were analyzed by flow cytometry. Data are representative of two independent experiments. Results are expressed as mean ± SD. *P < 0.05, Student’s t test.
