Fig 4. Western blotting analysis for GST pull-down samples.

1, Cell lysates from the CHO cells (which do not express endogenous chicken SAV1, STK4, STK3, LATS1 and MOB2) cotransfected by empty pSF-GST and each of the pcDNA3.0-STK4, pcDNA3.0-STK3, pcDNA3.0-LATS1 and pcDNA3.0-MOB2 expression constructs, respectively. 2, Cell lysates from GST-SAV1 and each of the pcDNA3.0-STK4, STK3, LATS1 and MOB2 transfected cells, respectively. The co-transfection of CHO cells with empty pSF-GST vector and the pcDNA3.0-STK4, STK3, LATS1 and MOB2 for 24 h was used as a negative control. GST, glutathione S-transferase