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. 2016 Aug 9;11(8):e0160937. doi: 10.1371/journal.pone.0160937

Fig 4. B. infantis-induced IL-1ß secretion by THP-1 macrophages involves caspase 1.

Fig 4

A. THP-1 macrophages were infected with B. infantis or C. rodentium for 1 hour, washed, then incubated in fresh medium for 4 hours. ZYVAD at a final concentration of 50 or 100 μM was present in the medium, as indicated, throughout the course of the experiment. Supernatants were collected at the end of the 4 hour incubation and IL-1ß concentrations determined by ELISA. *p < 0.0001, **p = 0.0002, n = 6 per experimental condition. B. THP-1 macrophages were infected with B. infantis for 1 hour, washed, then incubated in fresh medium for the times indicated before preparation of cell lysates. The lysates were analyzed by western blotting with an antibody to caspase 1, with equal loading of lanes being confirmed by blotting with an anti-ß-actin antibody. The p10 processed form of caspase 1 is shown.