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. 2016 Aug 9;11(8):e0160887. doi: 10.1371/journal.pone.0160887

Fig 2. Effect of miR-17-3p on ARPE-19 cell viability.

Fig 2

(A) ARPE-19 cells were transfected with miR-17-3p mimic (mimic), scrambled miR-17-3p mimic (sc-mimic), miR-17-3p antisense (inhibitor) or scrambled miR-17-3p antisense (sc-inhibitor) as indicated for 72 hours. Cell viability was assessed by MTT assay. The mimic sequence transfection reduced cell viability compared to scrambled sequence (sc-mimic); however, decreased cell viability was reversed by co-transfection with miR-17-3p inhibitor. Data shown as mean ± SD. (n = 8) (B) After 72 hours of transfection, cell death was evaluated by TUNEL staining. Representative images were chosen from three independent experiments. (C) The average number of TUNEL positive cells from different transfection groups was counted from six random microscope fields. Results are displayed as mean ± SD. *p<0.05.