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. 2016 Aug 9;11(8):e0160735. doi: 10.1371/journal.pone.0160735

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© 2016 Zach et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — Heart tissue slices were stained using the Trichrome (Gieson) protocol and scanned with a high resolution scanner. Sample images are cut-outs with a size of 1024 x 768 pixels. (A) Micrograph image section with fibres predominately oriented parallel to the image plane. Details of the green region of interest (ROI) are depicted in Fig 2. (B) Tissue with myocytes oriented rather oblique and perpendicular to the image plane. (C) Region with cells mixed in orientation from parallel to perpendicular to the image plane.