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(A) shows the unprocessed image. (B) Generation of an unsharp mask, multiplied with (A). (C) Noise removal. (D) Colour deconvolution to extract the cyan coloured parts of the image. (E) Application of a Gaussian blur. (F) Adding (E) to (A) generates a mask around the nuclei. (G) Thresholding and removing noise and (H) Gaussian blur extracts the shape of the intersected nuclei from the generated mask.
