Extended Data Fig. 8. Metagenomic analysis of the occurrence of the yeast mannan PULs in humans.

Abundance of Bacteroides mannan PULs in human from a survey of metagenomic sequencing data from a total of 250 adult human samples (211 healthy, 27 ulcerative colitis, 12 Crohn’s Disease; see Methods for references). Datasets were individually queried by BLAST using either each entire mannan PUL (PULs 2,3) or a sub-fragment that was trimmed to eliminate cross-detection of other species genomes beyond Bt and porcine B. xylanisolvens (PUL1; see Methods for additional search details). Each horizontal line represents the presence of a hit in a single individual. The leftmost column summarizes the total mannan PUL content in each person (annotated according to the color key in the upper right corner). The mannan PUL frequency across all 250 samples is shown at the bottom for each condition and is compared to the frequency of several other PULs implicated in xyloglucan and porphyran utilization. Graph at far right illustrates the variation in sequencing depth for each sample/study: black lines show the average depth in megabasepairs (Mbp) for each study; the light gray line shows the depth for each individual sample.