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. Author manuscript; available in PMC: 2016 Aug 9.
Published in final edited form as: Nature. 2015 Jan 8;517(7533):165–169. doi: 10.1038/nature13995

Extended Data Fig. 1. The role of specific Bt PULs and enzymes in utilization of mannan from S. cerevisiae and other yeast species.

Extended Data Fig. 1

a, growth of wild type Bt on Candida albicans mannan and glucose. b, growth of wild type Bt and the mutant lacking PUL-Man1 and PUL-Man3 (ΔPUL-Man1/3) on Schizosaccharomyces pombe α-mannan. c, growth of wild type Bt, and the Bt mutants lacking Pul-Man2 (ΔPUL-Man2), or all three mannan PULs (ΔPUL-Man1/2/3) on S.cerevisiae α-mannan. d, displays the growth profile of wild-type Bt and the Bt mutant lacking bt3774 (Δbt3774) on S. cerevisiae mannan. In panels a, b, c and d, each point on the growth curve represents the mean of three biological replicates. e, enzymes at 1 µM at 37 °C were incubated with either undecorated α-1,6-mannan (derived from mnn2 mutant of S. cerevisiae), lanes 1-3, or mannan from S. pombe, lanes 4-9. Lanes 1 and 4, the mannans incubated in the absence of the enzymes; lanes 2 and 6, mannans incubated with the periplasmic mannanase BT3782, and in lanes 3 and 7 the surface mannanase BT3792; lane 5, S. pombe mannan incubated with the GH97 α-galactosidase BT2620; lanes 8 and 9, S. pombe mannan incubated with BT2620/BT3782 and BT2620/BT3792, respectively. Lane 10 galactose standard; lane 11 α-1,6-mannooligosaccharides: mannose (M1), mannobiose (M2), mannotriose (M3) and mannotetraose (M4).