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. 2016 Aug 9;11(8):e0159745. doi: 10.1371/journal.pone.0159745

Fig 7. H3S10ph, transcriptionally activating histone modification, is strongly induced during epileptogenesis in the hippocampal chromatin.

Fig 7

(A) The phosphorylation of the histone H3 on serine 10 (H3S10ph) gradually and strongly increases in chromatin of the Mmp-9 proximal promoter during epileptogenesis in the rat hippocampus in vivo. DNA was isolated from the hippocampal samples obtained by chromatin immunoprecipitation with anti-H3S10ph antibody from the unstimulated (control), as well as from the partially kindled and fully kindled rats. Mmp-9 proximal promoter content was evaluated by qPCR. Control ChIP reaction was performed using isotype antibody. Values are means ± SEM (*, p < 0.05; **, p < 0.01; n = 4). (B) During epileptogenesis in the rat hippocampus, the histone H3 is strongly phosphorylated on serine 10. Equal amounts (20 μg) of nuclear cell lysates obtained from the unstimulated (control) as well as the partially kindled and fully kindled rat hippocampi were analyzed by Western blot with anti-H3S10ph antibody. As a loading control, histone H3 was used. Representative Western blot analyses are shown.