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. 2016 Jun 22;5:e15258. doi: 10.7554/eLife.15258

Figure 2. Ubr3 genetically interacts with MyoVIIa.

(A) The normal filamentous structure of NompA in the apical junction of wild-type cells (white box) collapses into puncta in the detached scolopidia in flies in which myoVIIa is knocked down (yellow box). Arrow indicates detached scolopidia. (B) A diagram shows actin (cyan) and NompA (red) in a single scolopidium. (C) A diagram illustrates the detachment of scolopidia and altered NompA pattern. (D–E) The normal filamentous structure of NompA in the apical junction of wild-type cells (white boxes) collapses into puncta in the detached scolopidia in ubr3 mutant cells (labeled by GFP) (yellow boxes). (F) The normal filamentous structure of NompA in the apical junction of wild-type cells (white box) collapses into puncta in the detached scolopidia in cells over-expressing ubcD6 RNAi construct (labeled by GFP) (yellow box). (G) Immunolabeling of Johnston’s organ with ubr3 mutant clones (marked by GFP, green) by anti-HRP (neurons, blue) and anti-MyoVIIa antibody (red). Arrows indicate detached ubr3 mutant scolopidia. (H) A diagram shows localization of MyoVIIa (red) in neuronal cilia and scolopale cells. (I) Quantification of detached scolopidia in the ubr3A/Aand ubr3B/B clones, ubcD6 RNAi clones, and wild type or mutant clones over-expressing myoVIIa. Error bars show SEM. Numbers of flies quantified are shown in the columns. (***p<0.001)

DOI: http://dx.doi.org/10.7554/eLife.15258.005

Figure 2.

Figure 2—figure supplement 1. ubr3 mutants phenocopy myoVIIa mutants.

Figure 2—figure supplement 1.

(A) Immunolabeling of pupal Johnston’s organs bearing ubr3 mutant clones over-expressing wild type Ubr3 or E3 ligase-dead form of Ubr3 (labeled by GFP, green) with anti-NompA antibody (red) and phalloidin (actin, blue). (B) Quantification of detached scolopidia in cells with indicated genotypes in Johnston’s organ. Numbers of flies quantified are shown in the columns. (C) Immunolabeling of a pupal Johnston’s organ bearing myoVIIa RNAi expressing clones (labeled by GFP, green) with anti-MyoVIIa antibody (red) and anti-HRP (neurons, blue). (D–D’) Enlargement of the ciliary region of the scolopidia from a wild type Johnston’s organ, labeled by anti-MyoVIIa (red) and anti-HRP (cyan).
DOI: http://dx.doi.org/10.7554/eLife.15258.006